The order mandates that federal departments define gender as an unchangeable male-female binary determined by sex assigned at birth (or conception, according to the EO). It also requires the replacement of “gender” with “sex” in all materials, the cessation of funding for gender-affirming care and the promotion of “gender ideology,” the prohibition of gender self-identification on federal documents and the use of federally funded facilities congruent with one’s gender identity. Additionally, the order directs the Attorney General to re-evaluate the application of Bostock v. Clayton County to potentially remove Title VII protection based on gender identity in federal activities.